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KMID : 0613820040140010167
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Journal of Life Science
2004 Volume.14 No. 1 p.167 ~ p.173
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Continuous Production of γ-aminobutyric Acid by Immobilization of Lactobacillus brevis
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Ryu Beung-Ho
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Abstract
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The optimal conditions for the continuous production of γ-aminobutyric acid by immobilization of Lactobacillus brevis BH-21 using column type reactor were investigated. The optimal conditions of operation were 2.2 mm diameter bead of 3.0% sodium alginate at 10 mL/h of substrate feeding rate. Continuous production by immobililzed cells showed the highest productivity with replacement of fresh medium in every 48h for fourth fermentatoin cycle following the condition of γ-aminobutyric acid productivity. A productivity of γ-aminobutyric acid could be obtained for 25 days by continuous column type reactor under optimal conditions.
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KEYWORD
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Lactobacillus brevis, γ- aminobutyric acid, immobililzed cells
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